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UID:DSC-21647
DTSTART;TZID=Europe/Berlin:20250204T110000
SEQUENCE:1738651067
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URL:https://dresden-science-calendar.org/calendar/de/detail/21647
LOCATION:MPI-CBG\, Pfotenhauerstraße 10801307 Dresden
SUMMARY:Lebek: PUFFFIN - Illuminating Cellular Neighbourhoods in Model Syst
 ems of Development
CLASS:PUBLIC
DESCRIPTION:Speaker: Tamina Lebek\nInstitute of Speaker: Centre for Regener
 ative Medicine\, Institute for Regeneration and Repair\, University of Edi
 nburgh\, UK\nTopics:\n\n Location:\n  Name: MPI-CBG (MPI-CBG CBG Galleria 
 II (VC))\n  Street: Pfotenhauerstraße 108\n  City: 01307 Dresden\n  Phone
 : +49 351 210-0\n  Fax: +49 351 210-2000\nDescription: Understanding how c
 ells influence their neighbours is crucial for deciphering processes like 
 development\, homeostasis\, and disease. Recent advances have introduced f
 luorescent protein-based methods to identify cellular neighbourhoods. I wi
 ll present Positive Ultra-bright Fluorescent Fusion For Identifying Neighb
 ours (PUFFFIN)\, a new neighbour-labelling system based on the secretion a
 nd uptake of a super-positive fluorescent protein. PUFFFIN enables precise
  and rapid identification\, isolation\, and profiling of neighbouring cell
 s\, even capturing transient interactions. Its modular design allows colou
 r-of-choice labelling and transgene integration\, making it highly versati
 le. I will also demonstrate how we are using PUFFFIN to study coordination
  of differentiation in mouse embryonic stem cells. This powerful tool offe
 rs a simple\, sensitive\, and customisable approach for profiling cell-cel
 l communication in diverse contexts\, paving the way for new insights into
  cellular interactions.
DTSTAMP:20260426T150248Z
CREATED:20250117T063851Z
LAST-MODIFIED:20250204T063747Z
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