The PoL PhD and Po stdoc seminar is a meeting where PoL early career scientists present their research to their peers. All are welcome!&\;nbsp\;
The seminar c onsists of two short talks by PhD students or Postdocs\, each followed by a discussion in which speaker and attendants can exchange ideas\, engage i n scientific discussions and network with their fellow young scientists.
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The talks:
Upon injury\, zebrafish larval pectoral fins can regenerate within 3.5 da ys\, re-acquiring morphologies strikingly similar to uninjured controls. T his project seeks to uncover the mechanisms underlying shape re-acquisitio n during the regeneration&\;nbsp\;of pectoral fins in zebrafish larvae. We&\;nbsp\;hypothesise&\;nbsp\;that the shape of the fin arises fro m a combination of factors\, including mechanical interactions within and between the tissues of the fin and directional BMP diffusion.&\;nbsp\; Our&\;nbsp\;research has identified two distinct shape changes in the p ectoral fin: 2D anisotropic growth- with the proximal-distal axis growing more than the anterior-posterior axis and 3D anisotropic growth marked by a transient increase in thickness. The latter is&\;nbsp\;interestingly a regeneration specific phenotype. To investigate the possible contributio ns tothickness&\;nbsp\;increase during fin regeneration\, we are studyi ng changes in individual tissue shape\, cell shape\, cell number and inter cellular spaces using 3D volumetric tissue and cell segmentation pipelines . From this we identified that&\;nbsp\;the cartilage plays a key role i n the overall thickness increase of the fin. Additionally\, the regenerati ng cartilage cells are larger and have different shapes when compared to d evelopmental age-matched controls. This could point towards either a chang e in&\;nbsp\;tissue mechanics or in the dynamics of BMP signaling gradi ents upon amputation.
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Abstract: In the&\;nbsp\;Brugués&\;nb sp\;lab\, we use advanced fluorescence microscopy techniques to address co mplex biological questions. Among these\, light-sheet microscopy stands ou t as a powerful tool for volumetric&\;nbsp\;imaging of living biologica l samples due to its ability to minimize phototoxicity and improve imaging speed and resolution. Recently\, I have constructed a SCAPE 2.0 microscop e in our lab—a specialized type of light-sheet microscope that operates with an oblique&\;nbsp\;plane\, enabling rapid\, high-resolution imagin g of dynamic biological processes. In this presentation\, I will talk abou t some technical details and unique advantages of the SCAPE 2.0 setup\, in cluding its ability to capture volumetric data with minimal sample&\;nb sp\;disturbance. Additionally\, I will discuss the challenges associated w ith the large and complex datasets it generates.
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For th ose who cannot join on site\, a Zoom option will be avail able:
https://tu-dresden.zoom-x.de/j/63359118163?pwd=XNnQgKbbSzWItRf
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Meeting ID: 633 5911 8163
Passcode: @T206x=%