BEGIN:VCALENDAR
VERSION:2.0
PRODID:www.dresden-science-calendar.de
METHOD:PUBLISH
CALSCALE:GREGORIAN
X-MICROSOFT-CALSCALE:GREGORIAN
X-WR-TIMEZONE:Europe/Berlin
BEGIN:VTIMEZONE
TZID:Europe/Berlin
X-LIC-LOCATION:Europe/Berlin
BEGIN:DAYLIGHT
TZNAME:CEST
TZOFFSETFROM:+0100
TZOFFSETTO:+0200
DTSTART:19810329T030000
RRULE:FREQ=YEARLY;INTERVAL=1;BYMONTH=3;BYDAY=-1SU
END:DAYLIGHT
BEGIN:STANDARD
TZNAME:CET
TZOFFSETFROM:+0200
TZOFFSETTO:+0100
DTSTART:19961027T030000
RRULE:FREQ=YEARLY;INTERVAL=1;BYMONTH=10;BYDAY=-1SU
END:STANDARD
END:VTIMEZONE
BEGIN:VEVENT
UID:DSC-21346
DTSTART;TZID=Europe/Berlin:20241024T110000
SEQUENCE:1729748566
TRANSP:OPAQUE
DTEND;TZID=Europe/Berlin:20241024T120000
URL:https://dresden-science-calendar.org/calendar/en/detail/21346
LOCATION:MPI-CBG\, Pfotenhauerstraße 10801307 Dresden
SUMMARY:Bezjak: Microstructured scaffolds for muscle tissue engineering and
  their effect on cell organization\, myogenic gene expression\, and transc
 riptomic profiles
CLASS:PUBLIC
DESCRIPTION:Speaker: Dragica Bezjak\nInstitute of Speaker: Universidad Téc
 nica Federico Santa María\, Valparaíso\, Chile\nTopics:\n\n Location:\n 
  Name: MPI-CBG (MPI-CBG: Galleria)\n  Street: Pfotenhauerstraße 108\n  Ci
 ty: 01307 Dresden\n  Phone: +49 351 210-0\n  Fax: +49 351 210-2000\nDescri
 ption: Muscle tissue engineering holds significant promise for regenerativ
 e medicine\, disease modeling\, and cultured meat production. This field u
 tilizes muscle cells\, scaffolds to support these cells\, and biochemical 
 cues to guide their development. For muscle cells to mature into functiona
 l tissue\, they must undergo myogenesis\, which involves cell proliferatio
 n\, followed by exit from cell cycle\, differentiation\, alignment\, and f
 usion into elongated\, multinucleated myofibers. An essential aspect of ef
 fective scaffold design is creating structures that promote proper cell al
 ignment and fusion. In this study\, we fabricated scaffolds from marine-de
 rived biopolymers\, including salmon gelatin\, alginate\, agarose\, and gl
 ycerol\, resulting in both flat and microstructured surfaces for comparati
 ve analysis. We assessed the impact of these microstructured scaffolds on 
 muscle cell behavior\, focusing on alignment\, glycolytic metabolism\, myo
 genic gene expression\, and transcriptomic profiles. Muscle cells cultured
  on microchannel scaffolds developed parallel\, elongated\, multinucleated
  structures resembling muscle bundles\, with elevated glycolytic metabolis
 m\, when compared to the use of flat scaffolds. Gene expression analysis r
 evealed significant correlations between myogenic and fusion markers\, suc
 h as Myomaker with MyoD\, Myomixer with Myosin heavy chain\, and Myogenin 
 with Myosin heavy chain. Immunofluorescence confirmed the expression of th
 ese markers after seven days of culture. Transcriptome analysis using high
  throughput sequencing highlighted notable differences between flat and mi
 crostructured scaffolds. Functional enrichment analysis identified key gen
 e modules related to muscle development\, including filament sliding\, mus
 cle contraction\, and sarcomere organization. This study enhances the unde
 rstanding of scaffold design in muscle tissue engineering\, providing insi
 ghts into the underlying mechanisms that are benefit by muscle cell alignm
 ent. The findings suggest that microstructured scaffolds are essential too
 ls for advancing muscle tissue engineering practices.
DTSTAMP:20260415T140120Z
CREATED:20241010T054117Z
LAST-MODIFIED:20241024T054246Z
END:VEVENT
END:VCALENDAR